Scintillation proximity assay for DNA binding by human p53.

Lipopolysaccharide affinity measurement by scintillation proximity assay: application to human heparin binding protein.

Scintillation Proximity Assay (SPA): Custom Assay Development and Membrane Validation

G protein-coupled receptors (GPCRs) represent attractive drug targets because they participate in a wide range of physiological processes and are believed to play a role in a variety of disease states. At least a third of all prescription drugs on the market today are molecules acting as agonists or antagonists of the GPCR. Receptor-ligand interaction studies are pivotal in the successful ident...

Functional Stability of the Human Kappa Opioid Receptor Reconstituted in Nanodiscs Revealed by a Time-Resolved Scintillation Proximity Assay

Long-term functional stability of isolated membrane proteins is crucial for many in vitro applications used to elucidate molecular mechanisms, and used for drug screening platforms in modern pharmaceutical industry. Compared to soluble proteins, the understanding at the molecular level of membrane proteins remains a challenge. This is partly due to the difficulty to isolate and simultaneously m...

Production and Evaluation of Polyclonal Rabbit Anti-Human p53 Antibody Using Bacterially Expressed Glutathione S-transferase-p53 fusion protein

p53 is a key tumor suppressor gene that is targeted for inactivation during human tumorigenesis. In this study, we produced and characterized polyclonal antihuman p53 antibody. The cDNA encoding the completehuman p53 protein was cloned into pGEX-4T-1 and expressed in Escherichia coli as a fusion protein with Schistosoma japonicum glutathione S-transferase (GST). The rabbits were immunized...

Analysis of mutant p53 for MAR-DNA binding: determining the dominant-oncogenic function of mutant p53

At least some mutant p53 proteins not s imply have los t the wild-type p53 specific tumor suppressor function, but exhibit oncogenic functions on their own. Recently we showed that binding of mutant p53 to MAR/SAR elements is an act ivity specif ic for mutant p53 and clearly distinguishable from the previously reported DNA-binding activities of p53. Since MAR/SAR elements are considered to be i...